{"product_id":"minute™-nuclear-proteasome-enrichment-kit","title":"Minute™ Nuclear Proteasome Enrichment Kit (20 preps)","description":"\u003ch2\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/41-PN-041_v4.pdf?v=1653411860\"\u003eManual \u0026amp; Protocol\u003c\/a\u003e \u003cspan style=\"color: rgb(246, 146, 30);\"\u003e|\u003c\/span\u003e \u003ca href=\"https:\/\/cdn2.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/msds-6_90daadfb-f6ff-472a-a06e-07d253025ac0.pdf?741\" target=\"_blank\"\u003eMSDS\u003c\/a\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe proteasome is a protein degradation system that requires metabolic energy and polymerization of ubiquitin that is different from lysosome-based protein degradation. The 26S proteasome is made up of two subcomplexes (a 20S proteasome and one or two19S regulatory particles). The proteasomes have been found mainly localized in the cytosol but also detected in the nucleus. Traditionally, ultracentrifugation and affinity isolation are the most common methods for the isolation of proteasomes. These methods, though relatively effective, are usually time-consuming with low yield. Many affinity-based methods require harsh elution conditions that may affect the activity of isolated proteasomes and limit certain downstream applications. In most cases, the methods can’t distinguish proteasomes derived from cytosol or nucleus. To overcome these shortcomings, we have developed spin-column-based kits for the enrichment of proteasome from nuclei (\u003ca href=\"https:\/\/inventbiotech.com\/products\/minutetm-cytosolic-proteasome-enrichment-kit-20-preps?_pos=1\u0026amp;_sid=4f0d31927\u0026amp;_ss=r\"\u003e\u003cstrong\u003eCytosolic Proteasome Enrichment Kit is also available under Cat # PT-040\u003c\/strong\u003e\u003c\/a\u003e). The protocol is simple and rapid with a high yield. The gentle protocol maintains the association of proteasomes with ubiquitin and other proteins and is useful for the studies of proteasome structure and function. The highly enriched proteasomes can also be used as a starting material for affinity purifications.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn2.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/N-Proteasome-Fresh-F_copy_4971dee6-855a-45a2-9a06-5eddb3bcb448_large.jpg?v=1563206141\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn2.shopify.com\/s\/files\/1\/0063\/4575\/2687\/files\/Protea-N-Frozen-F-2_large.jpg?v=1563382461\"\u003e\u003c\/p\u003e\n\u003cp\u003eKit Components:\u003c\/p\u003e\n\u003ctable style=\"width: 534.869px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 416px;\"\u003e\n\u003cp\u003e\u003cb\u003eItems\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 104.869px;\"\u003e\n\u003cp\u003e\u003cb\u003eQuantity\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 416px;\"\u003e\n\u003cp\u003e\u003cspan\u003eBuffer A\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 104.869px;\"\u003e\n\u003cp\u003e\u003cspan\u003e30 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 416px;\"\u003e\n\u003cp\u003e\u003cspan\u003eBuffer B\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 104.869px;\"\u003e\n\u003cp\u003e\u003cspan\u003e8 ml\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 416px;\"\u003e\n\u003cp\u003e\u003cspan\u003eFilter Cartridges with collection tube\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 104.869px;\"\u003e\n\u003cp\u003e\u003cspan\u003e40 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 416px;\"\u003e\n\u003cp\u003e\u003cspan\u003ePestle for 1.5 ml tube\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 104.869px;\"\u003e\n\u003cp\u003e\u003cspan\u003e2 units\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 416px;\"\u003e\n\u003cp\u003e\u003cspan\u003eProtein extraction powder\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 104.869px;\"\u003e\n\u003cp\u003e\u003cspan\u003e2.0 grams\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch5\u003eReferences (1)\u003c\/h5\u003e\n\u003cp\u003e1. Zhang, C. Y., Zhang, R., Zhang, L., Wang, Z. M., Sun, H. Z., Cui, Z. G., \u0026amp; Zheng, H. C. (2023). Regenerating gene 4 promotes chemoresistance of colorectal cancer by affecting lipid droplet synthesis and assembly. \u003cem\u003eWorld Journal of Gastroenterology\u003c\/em\u003e, \u003cem\u003e29\u003c\/em\u003e(35), 5104-5124.\u003c\/p\u003e","brand":"Cat #: PN-041","offers":[{"title":"20 preps","offer_id":41280675971136,"sku":"Cat #: PN-041","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/6342\/4320\/files\/Minute_Nuclear_Proteasome_Enrichment_Kit_20_preps.png?v=1782238041","url":"https:\/\/wearvico.com\/products\/minute%e2%84%a2-nuclear-proteasome-enrichment-kit","provider":"wearvico","version":"1.0","type":"link"}